Development and In Vitro Characterization of Nanoemulsion Containing Hippophaes rhamnoides Aqueous Extract

Objective: Hippophaes rhamnoides is a member of the Elaeagnaceae family and grows in many countries of the world such as Turkey, France, Germany, Norway, and Russia. Hippophaes rhamnoides extracts have antioxidant, anticancer, antidiabetic, antiviral, and cardiovascular protective effects. Nanoemulsions are suitable systems for formulations of herbal extracts. The aim of this study was to develop and in vitro characterize nanoemulsion formulation containing Hippophaes rhamnoides aqueous extract.

Methods: Hippophaes rhamnoides aqueous extract-containing nanoemulsion or blank nanoemulsion formulations were prepared using ethyl oleate, lipoid S100, Kolliphor RH40, Pluronic F68, Dimethyl sulfoxide (DMSO), and ultrapure water. The droplet size, polydispersity index, and zeta potential values of nanoemulsions were determined, and pH measurement, Fouriertransform infrared spectroscopy (FT-IR), and rheological analyses were also performed.

Results: The droplet size and polydispersity index values of nanoemulsions were found to be <200 nm and <0.3, respectively, which indicates monodispersity. The zeta potential values of blank nanoemulsion and Hippophaes rhamnoides aqueous extract-containing nanoemulsion were determined as (–)28.51 ± 2.61 and (–)30.11 ± 2.02 mV, respectively. FT-IR results showed that the extract is completely dissolved in the oil phase of formulations.

Conclusion: The nanoemulsion formulation containing Hippophaes rhamnoides aqueous extract was successfully prepared and characterized. The formulation may be beneficial for the usage of Hippophaes rhamnoides aqueous extract.

Cite this article as: Çetin S, Kilinboz YF, Kaplan ABU, Çetin M. Development and in vitro characterization of nanoemulsion containing hippophaes rhamnoides aqueous extract. Pharmata 2023;3(2):31-34.